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The fact that wild type SnCE1 shows less mitochondrial fragmentation compared to SnCE1 Y212A, although both are monomeric and active deSUMOylases, might be because the proteolytic processing is only triggered after SnCE1 deacetylation for wild type SnCE1 while SnCE1 Y212A is not acetylated when it is produced and autoproteolytic processing can start immediately following translocation into the host cell as supported by the more pronounced processing observed for SnCE1 Y212A in HEK-293T cells (Fig

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